FastQCFastQC Report
Wed 31 May 2023
EGAF00007942328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299403
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT41721.3934396114935388No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT16530.5520986763659683No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT15570.5200348693900863No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA11970.39979559323052877No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC11160.37274175609462834No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11090.3704037701693036No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC10530.3516998827667058No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT9700.3239780496521411No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC8610.28757226881494174No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7140.23847456438312242No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT6370.2127567192045504No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5370.17935692027133998No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5020.16766699064471632No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5010.16733299265538423No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG4590.15330507710343583No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT4490.1499650972101148No Hit
GTGCCATTCATTCCGCCGATGGCGGATCTGC4360.14562312334879746No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT4340.14495512737013322No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA4310.14395313340213692No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC3960.1322632037755133No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG3860.12892322388219224No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG3840.12825522790352803No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC3600.12023927615955753No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG3580.1195712801808933No Hit
TACTTCGATGGTTATGACCAGCAGTCTGTAG3510.11723329425556857No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG3440.11489530833024386No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG3370.11255732240491913No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG3360.11222332441558702No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA3330.11122133044759071No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA3060.10220338473562389No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG3010.10053339478896337No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)