FastQCFastQC Report
Wed 31 May 2023
EGAF00007942332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429622
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC56171.3074283905386597No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG41930.9759742285078511No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT40550.9438529684234047No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA33800.7867381093146999No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT29790.6934002448664174No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG29530.6873484132563044No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA25450.5923812095283761No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC25410.5914501585114357No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG24990.5816741228335607No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC21330.4964829547835074No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA19980.46505998296176637No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG18650.43410253664849563No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC17400.4050071923691058No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG15700.3654375241491357No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG15660.36450647313219525No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14230.33122139927657335No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG14180.33005758550539777No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC13570.31585905749705556No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC13480.3137641927089395No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC13230.30794512385306155No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT13220.3077123610988264No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC12830.2986346136836568No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC12750.29677251164977586No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG11440.26628059084497535No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC11140.2592977082179218No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA10010.23299551698935345No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9910.23066788944700223No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC9830.22880578741312133No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA9790.22787473639618083No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC9640.22438329508265406No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC9030.21018476707431183No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA8910.20739161402349043No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT8660.20157254516761247No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG8590.1999432058879666No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8330.19389137427785355No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG8310.19342584876938332No Hit
TCTATCCTGAAACCAGGTGCTTCTGCTGCCC8110.18877059368468094No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7700.17922732076104111No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC7420.1727099636424578No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT7130.16595984376963938No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG7100.165261555506934No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7010.16316669071881792No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC6940.1615373514391721No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG6650.15478723156635368No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC6570.15292512953247273No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6520.15176131576129714No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6520.15176131576129714No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC6270.14594224690541918No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG6140.14291633110036264No Hit
CTGCGTGTATTCGCTTGTTGCTGCGTTAAAC6010.1398904152953061No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5810.13523516021060375No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT5720.13314029542248768No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5700.13267476991401744No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5610.13057990512590137No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG5580.12988161686319602No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG5260.12243320872767224No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5240.121967683219202No Hit
CGTTCTCGCCACGAGAACACCTCTCAGGTTC5240.121967683219202No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC5220.12150215771073176No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC5180.12057110669379129No Hit
ATCCACTCCCAGCGTGAACGTGACATGTTCC4980.11591585160908892No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT4770.11102783377015145No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA4590.1068381041939193No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG4560.10613981593121395No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT4520.10520876491427347No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT4480.104277713897333No Hit
GCACCGAAGCCTTCCAAACAGAGCAAGAAGA4420.1028811373719223No Hit
AACGGCGGCTGGTCCCACGACAACGATAATG4410.10264837461768717No Hit
ATGAGCGACGAAGGTCCGGGTACCGGCCCGG4410.10264837461768717No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)