FastQCFastQC Report
Wed 31 May 2023
EGAF00007942378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511551
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA97151.8991263823157418No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA55941.0935371057822192No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC55271.0804396824559037No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA50870.9944267531487574No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT50750.9920809459858352No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG39260.7674699101360373No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA34320.6709008485957412No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG29790.5823466281954292No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT26030.5088446704238678No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC22960.4488311038391089No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG22900.4476582002576478No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG19750.3860807622309408No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC19590.38295301934704457No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC15290.2988949293423334No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG14810.28951170069064475No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC13760.2689858880150757No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT13680.2674220165731276No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC13070.2554974968282732No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC12380.24200910564147077No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG12100.23653555559465236No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT11730.2293026501756423No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA11290.22070135724492768No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA10900.21307748396543064No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC10740.2099497410815344No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT10440.20408522317422895No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC10440.20408522317422895No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG10060.19665683382497542No Hit
GTCCACATCAAACTGCCGAAGCTGGCTGCAA10000.19548393024351432No Hit
CAGCGTCTGAAGGGTAAAGAAGGTCGTGTAC9990.19528844631327083No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC9420.1841458622893905No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA9310.18199553905671184No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9060.177108440800624No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC9040.17671747294013695No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC8120.15873295135773363No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7960.1556052084738374No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7870.15384585310164578No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC7430.14524456017093115No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG6620.1294103618212065No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT6500.1270645546582843No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6270.1225684242626835No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG6100.11924519744854374No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6040.11807229386708265No Hit
AGCGTGCACATCAAGATCCCGAAAGTTTCCC6010.11748584207635213No Hit
ATGTCCAACATGGACATCGATGGTATTAACA5260.10282454730808853No Hit
GGCGCCGAAACCGAAGAAGGTGGTGACGATC5210.10184712765687097No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5160.1008697080056534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)