FastQCFastQC Report
Sun 21 May 2023
EGAF00007942380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491962
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA91391.8576638033018809No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA52411.0653261837296377No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC51201.0407307881503043No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT47820.9720262947138193No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA47500.96552172728788No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45150.9177538102536374No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG37060.753310215016607No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA31090.631959378976425No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG29100.5915091002963643No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT24820.5045105109744249No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG21650.44007463991121265No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC20570.41812172484866716No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG19510.39657534525024296No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC17920.3642557758526065No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG13860.28172907663600033No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT12970.26363824848260636No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC12930.26282517755436396No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC12890.2620121066261215No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC12080.2455474203292124No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG11690.2376199787788488No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC11030.22420430846284872No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT11020.22400104073078816No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA10190.20712981896975785No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC9940.20204812566824268No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT9700.19716970009878815No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9660.1963566291705457No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC9340.18985206174460628No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA9140.18578670710339415No Hit
CAGCGTCTGAAGGGTAAAGAAGGTCGTGTAC9100.18497363617515175No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG8970.18233115565836386No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC8810.17907887194539415No Hit
GTCCACATCAAACTGCCGAAGCTGGCTGCAA8670.17623312369654565No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC8140.16545993389733354No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC7710.15671942141872744No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7420.15082465718896987No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7180.14594623161951534No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6860.13944166419357593No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG6350.12907500985848502No Hit
AGCGTGCACATCAAGATCCCGAAAGTTTCCC5990.12175737150430317No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5970.12135083604018196No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5830.11850508779133347No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5830.11850508779133347No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5640.11464300088218195No Hit
GGCGCCGAAACCGAAGAAGGTGGTGACGATC4960.10082079510206073No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)