FastQCFastQC Report
Sun 21 May 2023
EGAF00007942586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421216
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60411.4341810377573503No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT53091.2603984653954265No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18000.42733419433259895No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC17340.411665273873737No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG17100.405967484615969No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG16040.38080224872749374No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC15930.3781907619843501No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG15850.37629149889842745No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG14000.3323710400364659No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG13680.3247739876927752No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13670.3245365798070349No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC13500.3205006457494492No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG12230.290349844260427No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG12140.28821317328876395No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG10660.2530768061991947No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC9920.23550862265441008No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC9200.21841525488110614No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT9040.2146167287092608No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8640.20512041327964747No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG8220.19514928207855353No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG7810.18541555876319987No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7650.18161703259135456No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7530.17876813796247054No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7420.1761566512193269No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7310.17354516447618326No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG7130.16927182253285725No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG6870.16309921750360862No Hit
GGTGCCCCGCGTGGCTCTCCAGCAACCCCGA6560.15573957304565827No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6190.14695548127326596No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5990.1422073235584593No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA5870.1393584289295753No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5620.13342323178606702No Hit
GCTCTGGCCGATTTCTCCCGTGACGAACACC5570.13223619235736536No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC5300.12582617944237637No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC5230.12416432424219404No Hit
CTGGAGGTTACTGGCGACATGCAATGGGCGG5170.12273987692775204No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG5160.1225024690420117No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA4990.11846653498442604No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4970.11799171921294538No Hit
GGTATGAACACCGCGAACACCGCTGGTACTT4400.1044594697257464No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)