FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007942610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459945
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA56691.2325386731022188No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA27540.5987672439095979No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA25380.5518051071323745No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC23140.5031036319559947No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT19690.42809466349237413No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19290.419397971496592No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14250.3098196523497375No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT13610.29590494515648613No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG13300.289165008859755No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13030.28329474176260205No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT12940.2813379860635511No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11760.2556827446759939No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11730.25503049277631024No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10880.23655002228527322No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10500.22828816488928025No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10210.2219830631923382No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10200.22176564589244369No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9440.20524193110045766No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9350.2032851754014067No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8630.1876311298089989No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA8250.1793692724130059No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8250.1793692724130059No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8200.17828218591353315No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC7960.1730641707160639No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA7670.16675906901912185No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT7620.1656719825196491No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA7300.1587146289230234No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG7140.15523595212471056No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC7100.15436628292513235No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6910.15023535422713585No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6760.14697409472871756No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6470.14066899303177555No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6450.14023415843198642No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6320.13740773353335725No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6170.13414647403493896No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6060.1317548837360989No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC5970.1297981280370479No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5810.12631945123873506No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5590.12153627064105492No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5530.1202317668416876No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5340.1161008381436911No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC5010.10892606724717087No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4980.10827381534748719No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG4970.10805639804759265No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT4750.1032732174499125No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC4680.10175129635065062No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4620.1004467925512833No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)