FastQCFastQC Report
Wed 31 May 2023
EGAF00007942697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489243
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA16200.33112379737676373No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA14900.30455213462430736No Hit
CAGAGCGAAAGTAATGTTTTTGCCATCACGCTTAACCTTCAGGT11410.23321744000425146No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC10850.2217711852801164No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10810.22095359565696393No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG10330.2111425201791339No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT9940.20317102135339699No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA9630.1968347017739651No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT9400.1921335614408382No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG8390.1714894234562375No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA8150.16658388571732247No Hit
CTGGACGGAAACAGAACGACGGCTACGTTTGGAGTGGCGGGTAC7410.15145847768900117No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG7170.14655293995008617No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7050.14410017108062864No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7010.14328258145747613No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG6840.139807825559078No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG6690.1367418644722561No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6570.1342890956027986No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG6350.12979235267545983No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA6160.12590880196548546No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5920.12100326422657044No Hit
GTTAGCGGACAGTTCCGCCGCTTTACGTTCCGCTTCCTGACGGT5880.12018567460341793No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5810.11875489276290106No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA5590.11425814983556228No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG5490.11221417577768104No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5420.11078339393716416No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG5320.1087394198792829No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5250.10730863803876602No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5160.10546906138667288No Hit
TGCGGTTTTGCGTTCGGTTTCGGCTTTCTGTTTGACTGCCAGCT4980.10178990808248661No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)