FastQCFastQC Report
Sun 21 May 2023
EGAF00007942700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473234
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT62811.3272503666262356No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG27870.5889264084998119No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT25370.5360984206544753No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG24760.5232083916202133No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24600.5198274003981117No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC18440.38965923834720245No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG16160.3414801134322555No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA16000.338099122210154No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12650.267309618497403No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12350.2609702599559626No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9120.19271649965978777No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG8900.18806763672939814No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8790.18574320526420332No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8690.1836300857503899No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8690.1836300857503899No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8670.18320746184762718No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8540.18046040647966965No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8270.17475498379237334No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8180.1728531762299412No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7970.16841562525093295No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7910.16714775354264486No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7870.1663025057371195No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7870.1663025057371195No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG7850.16587988183435678No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA7210.15235591694595063No Hit
TCCTACACCAACGGCAAACTGACTATCACCC7130.15066542133489988No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6930.14643918230727293No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA6890.14559393450174757No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT6830.14432606279345947No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG6110.12911160229400254No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA6000.12678717082880772No Hit
GATGGTGAACGTAACAACCAGGTTCTGGAAG5280.1115727103293508No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG5270.11136139837796948No Hit
TCTATCCTGAAACCAGGTGCTTCTGCTGCCC5200.10988221471830004No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA5150.1088256549613933No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5150.1088256549613933No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5110.10798040715586793No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4960.10481072788514773No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC4940.10438810398238503No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA4910.103754168128241No Hit
ATCGATGAAGACAACCCGACCGATAACGGCA4750.10037317690613945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)