FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007942804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402108
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA35320.8783709849095269No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG27730.6896157251285724No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20340.5058342534841386No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC19380.48196007042884004No Hit
CCGCACCTGCAGTCTGAAACCGGTCCGCCGC19270.4792244869537537No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC18410.4578371979667154No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT17680.43968287126841543No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC17680.43968287126841543No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC17330.43097874202950454No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC15690.39019367931003607No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC14460.3596048822704348No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12250.30464452336188286No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC12190.3031523869209267No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC11450.28474937081580076No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC11350.28226247674754046No Hit
GTTCACGGCCACACCCAGGGTCCGTGGGACC11280.2805216508997583No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC11190.278283446238324No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA10890.2708227640335432No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC10780.26808718055845693No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10110.25142499030111315No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA10080.250678922080635No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9600.23874183055298576No Hit
CCGCACCTGCAGTCTGAAACCGGTCCGCCAC9020.22431784495707618No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG8690.21611109453181732No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG8420.2093964805475146No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA7970.19820545724034336No Hit
TCCTGGGCCCCGAGCGTATGCGCGCTGTCTG7760.19298297969699682No Hit
GGTCTGCAATCTTCCTCTTACCCAGGTTATG7450.18527360808539No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7390.18378147164443384No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7330.18228933520347768No Hit
GTGGCCCCGCTGCGTGCGTCCATGGGTCCGG7170.17831030469426123No Hit
GTACACGGCCACACCCAGGGCCCGTGGGACC7090.17632078943965304No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT7050.1753260318123489No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG6910.17184438011678455No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6860.17060093308265442No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG6690.16637321316661197No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA6480.1611507356232654No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6430.15990728858913528No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6420.15965859918230924No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6060.15070578053657227No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT5970.14846757587513804No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5920.14722412884100788No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5780.14374247714544353No Hit
CGTGGCCAGAGCCGTGGCCGTGGCCGTGGTC5640.14026082544987914No Hit
GGCCTGCAGTCTTCTTCTTATCCGGGTTACG5570.13851999960209693No Hit
GTTGGCCACGGTGTTACCGTACGTGACGTGC5370.1335462114655764No Hit
GGTCTGCAGAGCTCTTCTTACCCGGGTTATG5330.1325514538382723No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5240.13031324917683806No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG5170.12857242332905588No Hit
CCGCACCTGCAGAGCGAAACCGGTCCGCCGC5140.1278263551085778No Hit
GGTCACGGTCAGGACCAGGTTAGCCAGTTCC5020.12484208222666549No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA4990.12409601400618739No Hit
GTAGGTCCAACCCCTCACCAGACCCCGCAGG4960.1233499457857093No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT4910.12210649875157918No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA4860.12086305171744903No Hit
GGCCGCCCTTCCCTGAAACTGCAGGCTGCCC4840.120365672903797No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT4710.11713271061505864No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC4690.11663533180140657No Hit
CCAATTTTCTATCCGCCGGTACTGCAGCCGA4660.1158892635809285No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT4580.1138997483263203No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT4550.11315368010584222No Hit
ACCCCGCTGCCGCCGGTTTCTCCGGGCGATC4430.11016940722392989No Hit
CAGCCTACTCCGTCCCCGCCGCGCATGCACC4340.10793120256249564No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG4270.10619037671471346No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT4180.10395217205327921No Hit
AGCCCGACCCAACCGATCTCTCACGAAGAAC4160.10345479323962715No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC4130.10270872501914909No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4040.10047052035771485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)