FastQCFastQC Report
Wed 31 May 2023
EGAF00007942825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475345
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT26990.567798125571953No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC25600.5385562065447201No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG20670.43484206208122517No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT19970.420115915808518No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG18760.3946607201085527No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC18340.38582503234492843No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA16900.3555312457267879No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG14350.3018859985904975No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA14210.298940769335956No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG13350.28084864677234433No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT13270.2791656586268921No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG11960.25160672774511145No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11040.23225236407241057No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG11040.23225236407241057No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG11020.2318316170360475No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT10990.2312004964815029No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG10820.2276241466724169No Hit
GGTACGCTCGACGTGGCAACGTGCCAGCAGCAGACGCAGACCTT10600.22299592927242318No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA10460.22005070001788174No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC10160.21373949447243581No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC10150.21352912095425428No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA9740.2049038067088115No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG9600.20195857745427007No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC9270.19501625135427952No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA8800.18512869599974754No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC8780.1847079489633845No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA7810.16430171769977595No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7730.16261872955432372No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA7650.16093574140887146No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7510.15799051215433002No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT7040.14810295679979804No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC6860.14431623347253048No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC6850.14410585995434896No Hit
CAGACCCATTGGTTCGTTCATGATGTCGTTGTCCAGGCCCGGAC6570.13821540144526606No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6550.137794654408903No Hit
CAGCGCGCGCAGGCCCTCAGCGATGTTTTCGAACTTCGGGTTGG6370.13400793108163545No Hit
CAGTGCACGCAGGCCTTCAGCGATGTTTTCGAACTTTGGGTTGG6290.1323249429361832No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6170.12980046071800483No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT5990.12601373739073723No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5850.12306850813619583No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG5680.1194921583271098No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5400.1136016998180269No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5380.11318095278166383No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT5320.11191871167257465No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG5320.11191871167257465No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG5250.11044609704530393No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG5230.11002535000894087No Hit
TTCCTGGCCTTCTTCACCCTCATCACCGTCACCACCTTCATCGC5130.10792161482712556No Hit
GGCTGGAATGGATTCGGAGTTGCGCGGGCCGGTCGGCAGGGTGT5010.10539713260894719No Hit
CCAGTTACCATCTTCGGTGGTGCGTTCGACGTGGCAACGTGCCA5010.10539713260894719No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG4900.10308302390895033No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC4850.10203115631804269No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)