FastQCFastQC Report
Sun 21 May 2023
EGAF00007942850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495021
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC43500.8787505984594592No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29140.5886618951519229No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG28570.5771472321376264No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA28360.5729049878692015No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG27230.5500776734724385No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT24980.5046250563107424No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG24490.49472648635108407No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG21910.44260748533900585No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG21780.43998133412521895No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG20370.41149769403722264No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC19840.4007910775502453No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC19530.3945287169635227No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG19330.39048848432692757No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC15880.3207944713456601No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT15530.31372406423161847No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC13600.2747358192884746No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13480.2723116797065175No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13360.2698875401245604No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC12800.2585748887420938No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG12590.25433264447366877No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC12160.24564614430498907No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG11270.22766710907214036No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC10400.2100920971029512No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT10240.206859910993675No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10120.20443577141171787No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9890.19978950387963337No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG9060.18302253843776323No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7780.15716504956355387No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA7630.15413487508610746No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7500.15150872387232056No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG6780.13696388638057777No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6570.13272164211215282No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA6430.12989347926653616No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG6270.12666129315726No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6230.12585324662994096No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6040.1220150256251755No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT5830.11777278135675052No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5570.11252047892917674No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA5530.11171243240185769No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5520.11151042077002793No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG5470.11050036261087913No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5260.10625811834245416No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5100.10302593223317799No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5000.10100581591488038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)