FastQCFastQC Report
Sun 21 May 2023
EGAF00007942860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578475
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT62541.0811184580146074No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34270.5924197242750334No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC32030.5536972211417952No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC21940.379273088724664No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20320.3512684212800899No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG20170.3486753965167034No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG20040.3464281083884351No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18670.3227451488828385No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG18290.31617615281559275No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17880.30908855179566963No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16740.2893815635939323No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG15380.2658714724058948No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14080.24339859112321188No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC13120.22680323263753835No Hit
GAAGTCTCCATTGCCGAACCGACCACTTACA12950.22386447123903366No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12890.22282726133367906No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC12080.20882492761139204No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC11550.19966290678075974No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10940.1891179394096547No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10880.1880807295043001No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10600.1832404166126453No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG9700.1676822680323264No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8740.15108690954665283No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC8600.14866675310082544No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8470.14641946497255715No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA8390.14503651843208437No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8330.14399930852672976No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8300.14348070357405246No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG8220.14209775703357969No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8200.1417520203984615No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG8100.14002333722287047No Hit
TCCATCTCTAGCAGCGAGGAAATCGTCCCAA7950.137430312459484No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7710.1332814728380656No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA7500.12965123816932453No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7310.12636674013570162No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7250.12532953023034704No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA7220.12481092527766974No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7220.12481092527766974No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7180.12411945200743334No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG7100.12273650546696055No Hit
TCCGAGCGTTATCTGGGTTACCTGGAACAGC7000.12100782229136955No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG6170.10665975193396429No Hit
AACGCTACCGTAGCCCTGCAGGGCGAGGTTC6140.106141146981287No Hit
AAGGAAAACGACGCTAGCTCCGAATCTGGCA5840.10095509745451402No Hit
GACGGCGTAGGTTCTGGTATCGCAGCATCTA5830.10078222913695492No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5790.10009075586671853No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)