FastQCFastQC Report
Sun 21 May 2023
EGAF00007942874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543095
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT58231.0721881070530939No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA45620.8400003682596968No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32610.6004474355315368No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG32420.5969489684125245No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG24550.4520387777460665No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA24180.44522597335641095No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG22920.4220256124619081No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA21720.39993003065761973No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG14410.2653311114998297No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG12890.23734337454773105No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG10650.19609828851305944No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8870.16332317550336498No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7840.14435780112135077No Hit
GACGCTAACGGCAACAACCTGACTTTCGAAC7250.13349414006757565No Hit
GATGTCCAGTCTGGTGCGATCGTTGAACCGG7020.12925915355508705No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA6940.1277861147681345No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6580.12115744022684798No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT6240.11489702538229961No Hit
TACGACGACTTCCATACCGATGAAGATAAAC6030.11103029856654914No Hit
AACACTAATCATTCTCCGCAGAAACACCCAA5910.10882074038612029No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC5890.10845248068938217No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA5890.10845248068938217No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG5820.10716357175079866No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT5700.10495401357036983No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA5460.10053489720951216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)