FastQCFastQC Report
Wed 31 May 2023
EGAF00007942895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007942895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500459
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA19580.3912408409080464No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA17880.3572720242817094No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG17760.3548742254610268No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG12620.25216850930845486No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG11170.22319510689187325No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT10940.21859932581889827No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG8230.16444903578514924No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA7880.1574554558914916No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG7540.15066169256622422No Hit
GACGTTCTCTTCTTCGTCGGAGTCCTTCAGGTGACGGTAACCGT6010.12008975760252089No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG5800.11589360966632631No Hit
CCAAACTTCGTCGGCCGCATCACGAGAAGCGGTCTGAGTGACGG5690.11369562741403391No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC5610.11209709486691218No Hit
GTCGAAAGCGACGGTTTTCATGCCGGTGGTGGATTTCACCGGGC5280.10550314811003497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)