FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007943041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514500
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA33610.6532555879494655No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA32370.6291545189504373No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT22930.4456754130223518No Hit
AATATCAACGGTCTGGACACCCAGGCCCGGTGCCACTTTCTTGA11910.2314868804664723No Hit
TACGGTATCAGGGGATTCCGGCTGCACCTCGGTTTCGCCTTTGT8440.1640427599611273No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8390.1630709426627794No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG7830.1521865889212828No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT7590.14752186588921282No Hit
CGGCAGGTGACGACGCAGGGCGTTCGCCAGAGCAGCACCACCCT7520.14616132167152576No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7520.14616132167152576No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7370.14324586977648202No Hit
ATCGCCGGTCGGGGTGCCGGTGGTCGGCTTCACCTGGACCAGCA6690.13002915451895042No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6290.12225461613216715No Hit
GTCCGGGTTACGCTTACGGATCTCAGAGCCAGTAACCAGACCCA6060.11778425655976675No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC6040.11739552964042761No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA5940.11545189504373178No Hit
TGGACCGGTAACCGGACGGCCACGACGGCCGTTATCGAACAGGG5910.11486880466472303No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA5820.11311953352769678No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5750.11175898931000972No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG5390.10476190476190476No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA5370.1043731778425656No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5240.10184645286686103No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5150.1000971817298348No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)