FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007943049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503256
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT51931.0318803948686155No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC46600.9259700828206717No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA33530.6662613063728996No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA27770.5518066351916321No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT25860.5138537841575659No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA24650.4898103549684455No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT23410.4651708077002559No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT22720.4514600918816666No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA21400.4252308964026261No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT18990.3773427440507416No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT14580.2897133864275836No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG14210.282361263452398No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA14110.2803742031888343No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG14090.2799767911361216No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG12610.2505682992353792No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA11890.23626146533772077No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA9130.18141860206336338No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG9060.18002765987886882No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA8740.17366906703546503No Hit
GTGCAGGGACAGATCCAGCGGTGCATCTGGGTCCTCGTAACGTG8490.16870141637655586No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG8480.16850271035019948No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC8310.16512470790214123No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA8020.15936223313780662No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC7630.1516126981099083No Hit
GCCCTGACCGCGTGGGCCGGTGGACGGACCCTCACCCGGGTCGT7490.14883081374091914No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG7490.14883081374091914No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC7390.14684375347735545No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG7350.14604892937192998No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG7100.1410812787130208No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC6530.12975503521070786No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC6510.12935762315799515No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6430.1277679749471442No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT6260.12438997249908595No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG6160.12240291223552227No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG6090.1210119700510277No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG6030.1198197338928895No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA5660.11246761091770392No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5480.1088909024432893No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG5300.10531419396887468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)