FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007943059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468619
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC31130.6642923142254155No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT30520.6512753430825468No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA22920.4890966862205757No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA20110.4291332617755575No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA19900.4246520094148978No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT16600.35423232946167355No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA16280.3474037544359064No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT14980.3196626683937271No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA14940.3188090965155062No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT13500.28808050889955383No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA13220.2821055057520075No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT12310.262686745522482No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC11440.24412155717117742No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT10710.22854387039364601No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG10450.22299565318521017No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT10320.22022154458099225No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC9580.2044304648339056No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC9130.19482778120392044No Hit
TTTCAGGATAGACGGGCTTGCAACCGGCGGAGCGTTGGATACAG8470.1807438452132756No Hit
GTCGAAAGCGACGGTTTTCATGCCGGTGGTGGATTTCACCGGGC8400.17925009442638903No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG8190.17476884206572932No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT7840.16730008813129643No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7470.1594045482577531No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC6960.1485215068104366No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6720.14340007554111123No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6470.1380652513022306No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG6400.136571500515344No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG5640.12035363482914693No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA5560.11864649107270513No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5410.11544559652937675No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5410.11544559652937675No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC5380.11480541762071107No Hit
GTCGTGACGTTTCAGCGGGAATGGGCCACCCAGATCGTCGATGG5260.11224470198604837No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA5210.11117773713827225No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG4900.10456255508206026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)