FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007943060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468619
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC42310.902865654188157No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT34790.7423941410826279No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG31880.6802967869420574No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA24540.5236663472885222No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG23710.5059547308154386No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT22510.48034757446881154No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC18430.39328324289027977No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG17850.3809064506560767No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG14740.3145412371244017No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG13660.29149479641243736No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12440.26546085412669995No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG11970.25543138455760434No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT11400.2432679852929565No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC11220.23942691184096251No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG11160.23814655402363116No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10970.23409208760208186No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10200.21766082894632954No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9760.20827153828589964No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA9530.2033634999861295No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA9460.2018697491992429No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9170.19568135308214138No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9020.192480458538813No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC8660.18479831163482488No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8550.1824509889697174No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC8210.17519562800483976No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7810.16665990922263074No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7340.15663043965353518No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7320.1562036537144247No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA7020.14980186462776796No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6520.1391322161500067No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6390.1363581075457888No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6220.13273042706334998No Hit
GTGAACGCAAAAATTGATCGCCTGAACCAGA6190.1320902481546843No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6190.1320902481546843No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6030.1286759606418007No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5890.12568845906802756No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5720.12206077858558871No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG5510.11757952622492898No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5510.11757952622492898No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5150.1098973793209409No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5000.10669648477761252No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG4790.1022152324169528No Hit
TCTATCCTGAAACCAGGTGCTTCTGCTGCCC4700.10029469569095577No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)