FastQCFastQC Report
Wed 31 May 2023
EGAF00007943063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454694
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT21270.4677871271668419No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC21000.46184906772466755No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT20670.4545914395175657No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA16620.3655205478849512No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT15860.348806010195868No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT15650.3441875195186213No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT15430.3393491007138867No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC15060.33121175999683306No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA14730.3239541317897311No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC14240.3131776535428222No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT13260.2916246970490044No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG10580.2326839588822373No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA10330.22718575569503885No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA10210.22454661816518362No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT10100.2221274087628163No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10030.22058791187040075No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9850.2166292055756179No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA9610.21135093051590742No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC9550.2100313617509798No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT8550.18803854900218608No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7890.17352329258798224No Hit
TTGTGGCACCGGTGGCAGCAGGGTGGCCATGACACGCTGTTCCA7700.16934465816571145No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG7370.16208702995860952No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7020.15438954549653175No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG5490.12074054199087737No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT5370.11810140446102213No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT5150.11326298565628753No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5070.11150356063638403No Hit
ACCTGCACCAACTACGACCGGTTCCTTCAGCGGAGTGTGCAGTT5050.11106370438140815No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5040.11084377625392022No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC4840.10644521370416149No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT4650.10226657928189067No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT4550.10006729800701132No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)