FastQCFastQC Report
Sun 21 May 2023
EGAF00007943066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488341
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA26870.5502302694223913No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT26320.5389676476068976No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT21930.44907144802504806No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20050.4105737589102697No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC19720.40381618582097345No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA17150.351189025701303No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC15280.31289611152862445No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG14140.28955176812923755No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13820.2829989699820412No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13070.26764084932454985No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12340.25269227855125825No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA12130.24839200476716064No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11260.23057658480447066No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10560.2162423388574787No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA10410.21317071472598043No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9950.20375106738938573No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9910.20293196762098614No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG9790.20047466831578753No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9520.19494574487909064No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9150.18736907202139488No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8620.17651600009010096No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7990.16361517873780823No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7950.16279607896940868No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7650.15665283070641212No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7570.15501463116961303No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7450.15255733186441442No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7260.14866660796451658No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA6970.1427281346436199No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6850.1402708353384213No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6770.13863263580162224No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG6480.13269416248072555No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6380.1306464130597267No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG6350.13003208823342705No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6350.13003208823342705No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6130.12552703950722957No Hit
TCTATCCTGAAACCAGGTGCTTCTGCTGCCC6090.12470793973883003No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5820.11917901630213314No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC5420.11098801861813773No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG5400.11057846873393797No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5230.10709729471823992No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)