FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007943068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424086
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA49491.1669802823012312No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT36530.8613818895224081No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35940.8474696170116438No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC35150.8288413199209594No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA22490.5303169640120164No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21350.5034356239064718No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA20190.4760826813429352No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG19910.46948024693104706No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19530.4605198002291988No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG18970.44731493140542244No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17580.41453856057497773No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15270.36006847667690045No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15080.3555882533259763No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13400.3159736468546474No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC12170.28697009568813875No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG11020.2598529543535981No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9250.21811613682130512No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8230.19406441146371256No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8140.1919422004027485No Hit
ATCAACTCCAACGCCCCGATGGATAAATGCG8100.19099899548676447No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG7990.18840518196780842No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7900.18628297090684437No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7540.17779412666298816No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7500.17685092174700415No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7220.170248487335116No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG6840.16128804063326777No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6190.14596096074852744No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6160.14525355706153942No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG6070.14313134600057537No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5970.14077333371061532No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5900.13912272510764326No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC5680.13393509806973114No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5520.13016227840579506No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA5460.12874747103181902No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5450.128511669802823No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT5200.12261663907792288No Hit
ATCGAGAAAACCGATGAACTGGCAACCCTGC5070.1195512231009748No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4950.11672160835302274No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA4940.11648580712402673No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG4900.11554260220804272No Hit
ATCCCTCTGGGCATCGACGTAGGTGGCGGTC4860.11459939729205869No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC4830.11389199360507067No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG4750.11200558377310263No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC4560.10752536042217851No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC4380.10328093830025042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)