FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007943073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386320
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA69331.7946262166079932No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA62371.6144646924829156No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT31150.8063263615655415No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT30750.7959722509836404No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG27000.6989024642783185No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC25970.6722406295299234No Hit
TTTAGCTTCACGACCACGAGAACGAGAGCGGGCGCGACGGGTTG22480.581901014702837No Hit
ATCGCTAATCTGTACTTTTTTAGCTTCGCGGCCACGAGAACGGG19710.5101987989231724No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC17430.45118036860633676No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA17200.4452267550217436No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT16640.43073100020708216No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT15220.3939739076413336No Hit
CTTTGGGTGTTTCTGTTTCTGACGCGCTTCGCTAGAGGTACGTT14760.3820666804721474No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC14610.37818388900393457No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG13160.34065023814454337No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC12870.3331435079726651No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT12650.3274487471526196No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG12160.31476496168979085No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG11610.30052805963967694No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG9780.2531580037274798No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG9610.24875750673017186No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT9380.2428038931455788No Hit
AATCAGCGGGTTGAATGCCTGTTTTACCTTTTTCGGGTGTTTCT8450.21873058604265894No Hit
TTTAGTGCCGTTGACTGCAATTTCAACACGGCGGTCCGGCGCCA8320.21536550010354108No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC7510.19439842617519154No Hit
ACGACATTTACGGGAAGCGACACGGTTTTTGTAACGCTTAATCT7160.18533857941602816No Hit
GATACGGTGGGCGGTCAGATCGTTCTCCCACGCGAAGTCATCCA6830.17679643818595983No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6660.1723959411886519No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6640.17187823565955684No Hit
ACCCAGGGTGATCTGCTGAACACGATCGGAGTAGCCGCACGCTT6530.16903085524953407No Hit
AGTACCCGGACGGAAGTTGGACTGGAACAGCAGGGTGTCGGTAG6340.16411265272313108No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6210.16074756678401325No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG5830.15091116173120728No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5730.148322634085732No Hit
ACCCAGCGTGATAGAACGCACGCGGTCAGAGTAACCACATTCCT5650.14625181196935183No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5620.14547525367570924No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA5460.14133360944294887No Hit
GCGCTGTTTAGCCAGCAGTTCCGCTGCTTTACGTTCTTCCTGCT5450.14107475667840133No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5160.1335680265065231No Hit
GATGAGCGGGTTGAACGCCTGTTTAACCTTTTTAGGGTGTTTCT5110.13227376268378546No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5000.12942638227376269No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC4910.12709670739283496No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT4630.11984882998550424No Hit
TTTCGGGTGTTTCTGCTTCTGACGGGCTTCGCTGGAGGTACGTT4460.1154483329881963No Hit
GATCAGTGGGTTGAACGCCTGTTTAACTTTTTTTGGGTGTTTCT4120.10664733899358045No Hit
GGGCACTATCGAGCAACGCCTTCATTGCATCCGAGTAAGTATGA4020.10405881134810518No Hit
GATCAGCGGGTTGAATGCCTGTTTAACTTTCTTCGGGTGTTTCT3880.10043487264443986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)