FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007943074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386320
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT152193.9394802236487885No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA62131.6082522261337753No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53041.3729550631600747No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT30210.7819942016980741No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA27340.7077034582729343No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA26640.6895837647546076No Hit
GAAATGGAGAACATCCTGCAGAACCTGACTC21580.5586042658935597No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA21530.557310002070822No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA19750.5112342099813626No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC17810.4610167736591427No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG15760.4079519569269No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15220.3939739076413336No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG15120.3913853799958584No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG13470.34867467384551665No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12530.3243425139780493No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11000.2847380410022779No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10250.2653240836612135No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9340.2417684820873887No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC9290.2404742182646511No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9160.23710913232553324No Hit
TTCGTGGCAAAAGGTGTAGCCGCGGACGCCA8260.21381238351625592No Hit
GCCCGCCCGGAAACTCCATCCCCGGCAATCC7810.2021640091116173No Hit
ACCGATGGTGACGGTACTACTGATACCCTGC6850.17731414371505488No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6770.17524332159867467No Hit
GACGCCGCGTCCAGCAGCTCTGCAGGTCAGT6580.17032511907227169No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6300.16307724166494098No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6240.16152412507765582No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6060.15686477531580037No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5890.15246427831849244No Hit
AACCCACTGGGCCGTTTTCGTCCGGCCGAAA5690.14728722302754194No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5640.1459929592048043No Hit
CCGCCGCGCACTCCAACCTGGGAATCTCCGG5600.14495754814661418No Hit
GATGCGGCCTCCAATTCTGCAAACCGTCAGG5590.1446986953820667No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5540.14340443155932905No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG5500.14236902050113895No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5390.13952164009111617No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT5290.13693311244564094No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5060.13097949886104784No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA4690.1214019465727894No Hit
GCACGCCCGGAAACCCCGTCCCCGGCAATTC4540.11751915510457651No Hit
ATAACCCAGGGTCGTCGTGGTAACGTTTACT4100.1061296334644854No Hit
CCGCCTCGTACCCCAACCTGGGAATCTCCGG4000.10354110581901013No Hit
CAGGGCCAGCAGCTGGCCGATATCGGTGTGG3890.10069372540898738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)