FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007943075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398755
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA68861.7268748981203996No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA64631.6207947235771336No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT32140.8060087020852403No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT30280.7593635189527403No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC27750.6959160386703616No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG25940.6505247583102406No Hit
TTTAGCTTCACGACCACGAGAACGAGAGCGGGCGCGACGGGTTG22570.566011711451894No Hit
ATCGCTAATCTGTACTTTTTTAGCTTCGCGGCCACGAGAACGGG19720.49453925342628935No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA18010.45165577861092654No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC17360.43535504256999913No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT17190.43109177314391045No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT15700.39372547052701534No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG15090.3784278567039912No Hit
CTTTGGGTGTTTCTGTTTCTGACGCGCTTCGCTAGAGGTACGTT14310.3588669734548783No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC14260.3576130706824993No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC13820.3465787262855638No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT13770.3453248235131848No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG12720.3189928652932251No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG12140.3044475931336284No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG9600.2407493322967737No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG9410.2359845017617334No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT9310.23347669621697534No Hit
TTTAGTGCCGTTGACTGCAATTTCAACACGGCGGTCCGGCGCCA8680.21767752128499956No Hit
AATCAGCGGGTTGAATGCCTGTTTTACCTTTTTCGGGTGTTTCT8440.21165878797758023No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC7510.18833619641133026No Hit
GATACGGTGGGCGGTCAGATCGTTCTCCCACGCGAAGTCATCCA7300.18306980476733833No Hit
ACCCAGGGTGATCTGCTGAACACGATCGGAGTAGCCGCACGCTT6940.17404170480620934No Hit
ACGACATTTACGGGAAGCGACACGGTTTTTGTAACGCTTAATCT6680.16752141038983837No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6660.16701984928088676No Hit
AGTACCCGGACGGAAGTTGGACTGGAACAGCAGGGTGTCGGTAG6640.16651828817193515No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6440.16150267708241903No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6440.16150267708241903No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA6400.1604995548645158No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6260.15698862710185452No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6200.1554839437749997No Hit
ACCCAGCGTGATAGAACGCACGCGGTCAGAGTAACCACATTCCT6110.15322691878471745No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG5890.1477097465862497No Hit
GATGAGCGGGTTGAACGCCTGTTTAACCTTTTTAGGGTGTTTCT5740.14394803826911262No Hit
GCGCTGTTTAGCCAGCAGTTCCGCTGCTTTACGTTCTTCCTGCT5610.14068789106092713No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5320.13341525498112874No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5030.12614261890133038No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT4770.11962232448495944No Hit
TTTCGGGTGTTTCTGCTTCTGACGGGCTTCGCTGGAGGTACGTT4670.11711451894020138No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC4660.11686373838572559No Hit
GGGCACTATCGAGCAACGCCTTCATTGCATCCGAGTAAGTATGA4500.11285124951411268No Hit
GATCAGTGGGTTGAACGCCTGTTTAACTTTTTTTGGGTGTTTCT4400.11034344396935462No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA4170.10457549121641108No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)