FastQCFastQC Report
Sun 21 May 2023
EGAF00007943076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398755
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT156373.9214555303381777No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA62851.5761557848804404No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48841.2248122280598361No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT31210.7826861105189903No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA28390.7119659941568132No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA26040.6530325638549987No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA22240.5577359531541923No Hit
GAAATGGAGAACATCCTGCAGAACCTGACTC21740.5451969254304021No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA19830.49729783952552314No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC18590.4662010507705232No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG16590.41604493987536206No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15740.3947285927449185No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG15320.3841958094569347No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG14560.36513648731677345No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12760.3199959875111284No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11050.2771125126957656No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10800.2708429988338704No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9370.23498137954383017No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9100.2282103045729834No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC9030.22645484069165278No Hit
TTCGTGGCAAAAGGTGTAGCCGCGGACGCCA8700.21817908239395115No Hit
GCCCGCCCGGAAACTCCATCCCCGGCAATCC8020.20112600468959635No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7800.19560883249112862No Hit
ACCGATGGTGACGGTACTACTGATACCCTGC7310.18332058532181414No Hit
GACGCCGCGTCCAGCAGCTCTGCAGGTCAGT6860.17203546037040288No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6800.17053077704354805No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6560.1645120437361287No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6400.1604995548645158No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6380.1599979937555642No Hit
AACCCACTGGGCCGTTTTCGTCCGGCCGAAA6060.15197301601233842No Hit
GATGCGGCCTCCAATTCTGCAAACCGTCAGG6040.1514714549033868No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT5970.14971599102205616No Hit
CCGCCGCGCACTCCAACCTGGGAATCTCCGG5950.14921442991310455No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5830.14620506325939486No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG5760.14444959937806423No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5660.14194179383330618No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5350.1341675966445562No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5240.1314090105453223No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA4670.11711451894020138No Hit
GCACGCCCGGAAACCCCGTCCCCGGCAATTC4630.11611139672229814No Hit
CCGCCTCGTACCCCAACCTGGGAATCTCCGG4110.10307080788955623No Hit
ATAACCCAGGGTCGTCGTGGTAACGTTTACT3990.10006144123584657No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)