FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007943078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422744
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT90912.1504740457581892No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA25480.6027288382567227No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22010.5206460647578677No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC16270.3848664913044301No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT15130.35789981643737107No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13160.3112995098688568No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13090.3096436614121076No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG11310.2675378006547698No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA11270.26659160153662737No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA9410.22259334254300472No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9200.21762579717275704No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC7790.18427227825823667No Hit
CGTCGCCAGCGTGCAATCGACAAACGCCAGC7630.18048748178566698No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7470.1767026853130973No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7020.16605794523399503No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6960.1646386465567814No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6540.15470355581628598No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC6170.14595121397346858No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6080.1438222659576481No Hit
GTTACCATGACCTTCAAGCTGGGCCCTCGTA6020.14240296728043447No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG5720.13530647389436634No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5570.13175822720133226No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5390.12750033116969137No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG5140.12158658668130122No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC4790.11330734439755502No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4740.11212459549987701No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC4670.11046874704312776No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4670.11046874704312776No Hit
GGTCGCTGGAACCCGCAGCCGGGCGTGTACC4460.10550120167288003No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)