FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007943095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400544
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG30020.7494807062395142No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG22880.5712231365343133No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT17300.43191259886554284No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC15480.38647439482304063No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG15180.37898458096988097No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG14810.3697471438843173No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT13440.33554366062155466No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC12860.32106335383877926No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT12310.3073320284413198No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA11370.28386394503475276No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA11120.27762243349045296No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA10450.26089518255172967No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA10000.24966046177199008No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG9920.24766317807781418No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC9570.23892506191579452No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA8400.2097147878884717No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG8260.20621954142366383No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC7620.19024127187025644No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG6750.16852081169609331No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA6720.16777183031077733No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA6370.1590337141487577No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA6340.15828473276344174No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG6010.15004593752496606No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5590.13956019813054246No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA4970.12408124950067907No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG4820.12033634257409923No Hit
AACGCTCTGGGTGTGTTTCGGACCAGAGGAAACGCTGGCCAGGG4510.11259686825916752No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT4300.10735399856195574No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG4260.10635535671486777No Hit
ACGGCGATAGTTGGTCGCTTCCATGAACTGGGAAGTGCCCAGCG4190.10460773348246385No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT4170.10410841255891987No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)