FastQCFastQC Report
Wed 31 May 2023
EGAF00007943104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373817
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54441.4563275613468623No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC38341.0256355382446491No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA27450.7343165238606055No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT24480.6548658835740483No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT23570.6305224213987058No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA21700.5804979441812438No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG20220.5409063793246428No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14650.3919029899656784No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11470.30683462763865743No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10090.26991816851561057No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9620.2573451715679062No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9520.2546700658343521No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9400.25145993895408714No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT9150.24477217462020184No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8180.2188236490047269No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7550.20197048288333597No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC6990.1869898907754329No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC6130.16398398146686746No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6130.16398398146686746No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6030.16130887573331337No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT5550.1484683682122536No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5540.14820085763889818No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5520.14766583649218737No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5380.14392068846521158No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA5170.13830296642474793No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC5110.13669790298461545No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5080.13589537126454923No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA5040.1348253289711276No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT4990.13348777610435053No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4850.12974262807737474No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC4750.12706752234382065No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG4440.11877469456980287No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG4390.11743714170302583No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT4230.11315697252933922No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT4130.11048186679578509No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG3890.10406161303525521No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA3890.10406161303525521No Hit
ATGTCCAACATGGACATCGATGGTATTAACA3860.10325908131518899No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)