FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007943106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372544
Sequences flagged as poor quality0
Sequence length31
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58041.5579367806218862No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC49491.328433688369696No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA39591.0626932657618966No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG33800.9072753822367292No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT29130.7819210616732521No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA20640.5540285174368665No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG20210.5424862566569318No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG17010.4565903624806735No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16030.4302847448891943No Hit
GCTGAACGTGGTAAAGCAGGTGACCCGCACC14770.39646323655729254No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT13720.3682786462807078No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13700.3677417969421062No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT12960.3478783714138464No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT12860.3451941247208384No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG12510.33579926129531007No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT12400.3328465899330012No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC11800.31674110977495273No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC11770.3159358357670503No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11570.31056734238103423No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11390.30573569833361963No Hit
CGTCACGCACATCGCCAGGGTGTAGGCGGCA10870.29177761552997766No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9990.2681562446315066No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9870.2649351485998969No Hit
GGCGAAGCTGAACGTGGCAAAGCCGGTGATC9730.26117720322968563No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8810.23648213365401136No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8580.23030836626009277No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8480.22762411956708467No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8380.22493987287407663No Hit
GATGGCGAACACCGTCGTCACCGTGCCCACC8350.2241345988661742No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8090.2171555574643532No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA7760.20829754337742656No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7570.20319747466071122No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC7450.19997637862910153No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7410.19890267995189828No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7360.19756055660539426No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7160.19219206321937812No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7080.19004466586497165No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC6970.18709199450266276No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT6900.1852130218175571No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6800.18252877512454904No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG6710.18011295310084177No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC6410.17206021302181757No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6380.17125493901391514No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6320.1696443909981103No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6290.16883911699020787No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC6180.16588644562789898No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT6030.16186007558838686No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG6030.16186007558838686No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT5980.16051795224188284No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC5660.15192836282425698No Hit
AACACTTCTAGCGGTCAGACCCAATCTACCA5580.14978096546985054No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5430.14575459543033845No Hit
GCTTCCGAACCGGAGGACAAATCTCCGCGCG5400.144949321422436No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG5380.1444124720838344No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5350.14360719807593195No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC5170.13877555402851743No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5070.13609130733550936No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA4980.1336754853118021No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA4900.13152808795739565No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC4630.12428062188627384No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4620.12401219721697301No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG4580.12293849853976979No Hit
GCTCCGACCTGCAACATCCGTGTTACCGTTT4220.11327521044494074No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG4160.11166466242913589No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC4020.10790671705892459No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA3970.10656459371242055No Hit
GCCGATGGCGAACCGCGTCGCCACCGTGTCC3940.10575931970451813No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA3920.1052224703659165No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG3770.1011961003264044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)