FastQCFastQC Report
Sun 21 May 2023
EGAF00007943109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473531
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT27660.5841222644346411No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT15030.31740266212771706No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG14820.31296789439339767No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC13490.2848810320760415No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA13240.27960154667804216No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT12760.2694649347138836No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA12310.25996186099748486No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG11510.24306750772388713No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT11370.24011099590100757No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC9870.20843408351301185No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC9060.19132855082349415No Hit
TTTGTCGATGGTCTGCTCCAGGTTACCAAAGAAGCCTGCTTTTG8690.1835149124344552No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA8200.1731671210543766No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG7690.16239697084245802No Hit
GTCGTGACGTTTCAGCGGGAATGGGCCACCCAGATCGTCGATGG7580.16007399726733837No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG7140.1507821029668596No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA6970.1471920528962201No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6910.1459249764007003No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6770.14296846457782067No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA6550.1383225174275813No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA6320.13346539086142195No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6050.12776354663158274No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5770.12185052298582352No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5340.11276980810126475No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA5170.10917975803062523No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT5060.10685678445550556No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA4950.10453381088038588No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT4930.10411145204854592No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG4900.10347791380078601No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA4890.10326673438486604No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT4820.10178847847342623No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)