FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007943170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539440
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC62031.1498961886400711No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA45380.8412427702802906No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA38310.7101809283701617No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG37960.7036927183746107No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA35630.6604997775470859No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA27800.5153492510751891No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC26870.498109150229868No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG26870.498109150229868No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT26530.4918063176627614No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG25390.4706732908201098No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT24420.4526916802610114No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA23540.43637846655791196No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA23530.4361930891294676No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT22680.42043600771170103No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG20650.3828043897375055No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA19370.35907607889663357No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC18710.3468411686193089No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG16940.31402936378466556No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA14800.2743585940975827No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC14530.2693534035295862No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG14470.2682411389589204No Hit
GAAGATGGCAACAAACCGGGTAAGGAAGACG12650.23450244698205547No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT12570.23301942755450097No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT12260.22727272727272727No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG12110.2244920658460626No Hit
GAGGATAACAACAAACCGGGTAAGGAAGATG12060.22356517870384102No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG11770.21818923327895598No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG11020.2042859261456325No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC10040.18611893815808986No Hit
GCTCAGCACGATGAAGCACAGCAGAACGCGT9750.18074299273320482No Hit
GTTAAAGTTATCGCGACTGAGGGTGGCGTAC9740.1805576153047605No Hit
AACGGTTTTGTAGCTTGGGATTCCCAGTACA9590.1777769538780958No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9490.17592317959365267No Hit
GTTACCATTATCGCGGGTGAAGGTAACGTCA9480.17573780216520837No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT9270.1718448761678778No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA9120.1690642147412131No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT9090.16850808245588017No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA8830.16368826931632804No Hit
GAACAGCAGAATGCGTTCTACGAAATTCTGC8010.1484873201838944No Hit
CAGAACGCATTCTACGAAATCCTGCACCTGC7860.1457066587572297No Hit
ACCGACGTTCTGGAATCCGCGACTGCAGACA7840.1453359039003411No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG7800.14459439418656386No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7650.14181373275989917No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC7590.1407014681892333No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7320.13569627762123684No Hit
CTGCACGACGACGCTAACTTTCTGGAACATG6730.12475900934302239No Hit
GGTAAAGAAGACAACAAAAAACCGGGCAAAG6660.1234613673439122No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6640.12309061248702358No Hit
GTTAAGGTGGTAAGCGGTGAAGGTAACGTTA6490.1203099510603589No Hit
ATGGAAACCCTGCTGATCGACAACGATGGCT6430.119197686489693No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA6330.11734391220524988No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC6160.11419249592169657No Hit
GAATGCGACAACTATCGCCAGGAATACGATA5990.11104107963814326No Hit
GAAGCGCAGAAACTGAACGACTCTCAGGCCC5920.10974343763903308No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5860.1086311730683672No Hit
GGTTCTCGTGAGCGTGCACGCGGCCGTGGCC5610.10399673735725938No Hit
ACCACCACTTCTACTCAGACGAATAAACCGA5540.1026990953581492No Hit
GTAACCGTCATTGCGCAGCAGGGTGCAGTTC5530.10251371792970487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)