FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007943198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412233
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT70121.700979785703716No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA45521.104229889407204No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC23630.5732195142067714No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA22200.5385303942188034No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20520.4977767427644075No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20140.488558654935437No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA20110.48783091115946564No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19030.4616321352244968No Hit
GAACCGATCTACGCTACCATCGACGACCTGG18430.4470772597050697No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15690.3806099948330192No Hit
CCGGATTTTGAACAGAACATTGCGACCACCA15580.3779416009877909No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13250.3214201677206822No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12560.30468206087334104No Hit
GTGAACGCAAAAATTGATCGCCTGAACCAGA10510.2549529028486317No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10220.24791804634757528No Hit
CTGCCGATCCAGCAGGTCAGCAAAATGGGCG9670.23457607712143377No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9630.23360575208680526No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9380.2275412206203773No Hit
ACTAGCCCGGAAGAACCGATCTACACCCAGG8670.2103179512557219No Hit
AACACCGATGCACACGCTTCCAGCGATGCCC8620.20910504496243632No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8040.1950353319603234No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8010.19430758818435206No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7190.1744159249744683No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG7170.17393076245715408No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7080.17174753112924002No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6900.1673810684734119No Hit
TTCAACAAAAAACAGAGCTCCGACGTAAAAG6880.16689590595609763No Hit
ACTGCACTGTTCGCTCGCGAATACCAGACCC6170.1496726365914422No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5810.14093971127978597No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5790.1404545487624717No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC5620.1363306673653007No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5590.13560292358932932No Hit
GAACGTGGTAACTTCTCCAAATTCACCCTGG5500.13341969226141528No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5360.1300235546402156No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA5080.12323127939781629No Hit
ACCCTGGGCGACATGGAAATGCTGGATGCAG4740.11498351660347425No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG4410.10697833506778934No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA4270.10358219744658968No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA4240.10285445367061831No Hit
CTGCACGACGACGCTAACTTTCTGGAACATG4240.10285445367061831No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC4170.10115638486001848No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA4150.10067122234270423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)