FastQCFastQC Report
Wed 31 May 2023
EGAF00007943210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374649
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58171.552653283473331No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC37280.995064713905549No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA24390.6510093447466829No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG23230.6200470306873901No Hit
CGTTCTCGCCACGAGAACACCTCTCAGGTTC22240.5936222971367867No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20470.5463780765463141No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19060.5087428499742426No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT16080.42920173282192137No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14790.3947695042559836No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG14350.38302517823349325No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11380.3037509775816831No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10600.28293149054181377No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG10510.2805292420372135No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10460.2791946595346578No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10240.2733224965234126No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9480.25303684248456554No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9200.2455631804702535No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8960.239157184457986No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8840.23595418645185223No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8700.2322173554446962No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA8670.2314166059431628No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7090.18924379886240186No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT6990.18657463385729042No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6840.18257088634962326No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA6710.17910097184297835No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6640.17723255633940035No Hit
TCCTCCAACAGCCCGCACGTGGTTAAAACCG6490.17322880883173317No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6470.17269497583071086No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6440.17189422632917745No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6310.16842431182253256No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6080.16228523231077624No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5970.15934915080515363No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5910.15774765180208675No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5630.15027398978777468No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5460.14573640927908524No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5320.1419995782719292No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5300.14146574527090688No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5290.14119882877039575No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5230.1395973297673289No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4990.1331913337550614No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC4930.13158983475199454No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG4860.12972141924841651No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG4760.12705225424330505No Hit
ATGAGCCACCAGAGCCTGGACGATGACGCTG4620.12331542323614902No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC4540.12118009123205987No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG4420.11797709322592612No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG4410.11771017672541498No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT4300.1147740952197924No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT4010.10703351670496918No Hit
ACCCAGGGCGAAGTAAACGTGACGGGTGTTA3980.10623276720343575No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG3980.10623276720343575No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC3930.10489818470088001No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG3840.10249593619627972No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)