FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007943216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403729
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57461.423231920421867No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT44451.1009860574791506No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21760.538975401816565No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC16570.4104238239016766No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC15100.3740132613708701No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC12810.31729204491131424No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC12560.31109977237206143No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12500.3096136269626408No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG10790.26725848279415154No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC10170.25190164689680455No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9780.24224170173557016No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9130.22614179313351282No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8700.21549108436599798No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8420.20855573912203482No Hit
TCCTCCAACAGCCCGCACGTGGTTAAAACCG8050.19939117576394066No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT7050.1746220856069294No Hit
CGCGATAACAACATTGAACGTGACAACAATA6780.16793443126453636No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6570.162732922331564No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6360.15753141339859164No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6290.15579757708760084No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6260.1550545043828905No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6230.15431143167818018No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5790.1434130320090952No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5780.1431653411075251No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5700.1411838138949642No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG5600.13870690487926307No Hit
ACCCAGGGCGAAGTAAACGTGACGGGTGTTA5390.1335053959462907No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA5140.12731312340703788No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5080.12582697799761722No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT5000.12384545078505632No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG4800.11889163275365405No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG4790.11864394185208396No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC4700.11641472373795293No Hit
CCGACCAAGTCTCACTTCGCCAACCTGAAGG4530.11220397841126102No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA4460.11047014210027023No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA4370.10824092398613921No Hit
ACCACTCCGACGAAAAGCCACTTCGCAAACC4180.10353479685630707No Hit
AGCCCGACCCAACCGATCTCTCACGAAGAAC4100.10155326964374617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)