FastQCFastQC Report
Wed 31 May 2023
EGAF00007943280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485322
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA65221.3438500624327765No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44770.9224803326451304No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA32370.6669798607934526No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA30940.6375148870234607No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC26920.5546832824392878No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC25240.5200670894787378No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG24370.5021408466955959No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG23630.4868932378915442No Hit
ACTAGCCCGGAAGAACCGATCTACACCCAGG20660.4256967539077149No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG16270.335241344921516No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT15820.3259691503785116No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC14650.30186144456670005No Hit
CCGGATTTTGAACAGAACATTGCGACCACCA14610.30103724949621075No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC14470.29815256674949825No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT14090.29032271357985007No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13960.2876440796007599No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13150.27095412942335195No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA12260.25261578910496535No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12110.24952505759063054No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC10670.21985403505301637No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA9800.20192779226987445No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC9800.20192779226987445No Hit
GTGAACGCAAAAATTGATCGCCTGAACCAGA9270.19100720758589143No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC9230.19018301251540215No Hit
GCACTGGGCCAGCGTCGTGCCGATGCAGTTA8780.1809108179723977No Hit
GAACCGATCTACGCTACCATCGACGACCTGG8740.1800866229019084No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT8620.17761403769044057No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA8270.17040233082365935No Hit
TCCTACACCAACGGCAAACTGACTATCACCC8250.1699902332884147No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7810.16092408751303258No Hit
CGTGGCGAATTTGAACTGGTTGCGCAGCGCA7680.1582454535339424No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC7490.1543305269491183No Hit
GCGCTGGGCCAGCGTCGCGCTGACGCAGTTA7010.1444401861032469No Hit
GAAGTTGGCCGTAAACGTGTATTCCAGGAAA6970.14361599103275763No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6920.142585747194646No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6850.14114340582128979No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT6670.137434528004088No Hit
TGGATCGAAGTATCCGGCCAGGAGCTGCCGA6600.13599218663073176No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6350.13084096744017373No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG6330.13042886990492908No Hit
GATTACGGTAAGAAACAGGACAAAGCCCTGG6230.1283683822287059No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT6140.12651394332010502No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG5980.12321716303814788No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5980.12321716303814788No Hit
TTCAACAAAAAACAGAGCTCCGACGTAAAAG5850.1205385290590577No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5790.11930223645332377No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5750.11847804138283449No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5620.11579940740374431No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG5580.11497521233325503No Hit
CGTACCGTTGAATATTCCCCATTCGCTGGTC5580.11497521233325503No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5520.1137389197275211No Hit
GTTGACTCCCTGCACGATGACGGCGATGACG5380.11085423698080862No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5360.11044213944556398No Hit
GAACGTGTCGACATGCTGAAGGCGGGTGCAA5210.10735140793122916No Hit
GCCGAGGGTCTGAAGGACGCGATTAAAGACC5080.104672773952139No Hit
GAACGTGGTAACTTCTCCAAATTCACCCTGG5050.10405462764927202No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT5000.10302438381116043No Hit
GTCGGTGACGGTGAGCCAACGACCGATGCTA4980.10261228627591579No Hit
CCGCCACAGACCTCCGGCCAGGTTTACCTGC4870.10034574983207026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)