FastQCFastQC Report
Sun 21 May 2023
EGAF00007943321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523445
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT33970.6489698058057676No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT31060.5933765725147819No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC27570.5267029009733591No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC24090.46022027147073713No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA19540.3732961438164468No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC19200.3668007144972251No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA19090.3646992520704181No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT16650.3180849946030624No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC15370.2936316136365807No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA13470.2573336262644595No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12920.24682631413042438No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG12020.22963253063836697No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT12020.22963253063836697No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10330.19734642608105912No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC10180.19448079549904956No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA10100.19295245918864445No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG9910.18932266045143234No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9880.18874953433503042No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT9800.18722119802462533No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG8090.1545530093897162No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA7890.15073216861370345No Hit
GGTACGCTCGACGTGGCAACGTGCCAGCAGCAGACGCAGACCTT7730.14767549599289323No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7060.13487567939325049No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC6480.12379524114281347No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6300.120356484444402No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6000.11462522328038284No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5750.1098491723103669No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC5600.10698354172835732No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)