FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007943417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421819
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT51071.210708858538852No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC39300.9316792273463264No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA29680.7036193248763095No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA27810.659287514313011No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA27670.6559685552334058No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT23990.5687273451409254No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT21740.5153869313615556No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC21260.5040076430886233No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT20360.4826714775768754No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT19770.4686844357413962No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA19120.45327498287180046No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT15700.3721975539271583No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT15270.36200360818265653No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT13010.30842612589760066No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG10200.2418098757998099No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT9600.2275857654586446No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA9280.21999957327668976No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG9140.21668061419708456No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG8990.2131245866117932No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA8110.19226255811141746No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA8090.19178842110004526No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG8020.19012894156024265No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA7700.18254274937828785No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG7370.17471948869064693No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC7050.16713329650869213No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6370.15101263812203813No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6150.1457971309969442No Hit
GCCCTGACCGCGTGGGCCGGTGGACGGACCCTCACCCGGGTCGT6100.14461178846851375No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6000.14224110341165286No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC5920.14034455536616416No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC5760.13655145927518675No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT5730.1358402537581285No Hit
CACGCAGGCACCACGACGGCGACGAGACGGCGGCGGAGTAGGTT5070.12019373238284668No Hit
CTGTTCGGTTTCCAGTTCGCCTACGCTCACTTTGGTGTTGTTAC4960.1175859788202997No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG4820.11426701974069446No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA4690.11118512916677532No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA4610.10928858112128663No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4600.10905151261560053No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA4570.10834030709854228No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG4550.10786617008717009No Hit
CAGGTACGGCGGATAGGTGTGGGCTTCCGCTTCGCCACGGTCTG4240.10051704641090135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)