FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007943546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473911
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT67781.4302263505172914No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC39900.8419302358459712No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG23550.49692874822487765No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA23050.48637824401628155No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG22920.48363511292204653No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19250.4061944120309509No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18330.38678148428713405No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16890.3563960321663772No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15450.32601058004562034No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG14830.3129279548269612No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA11940.25194604050127556No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC10790.22767988082150445No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9980.21058806400357874No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9770.20615685223596836No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9600.20256968080504567No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9450.19940452954246685No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG8870.18716594466049533No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8670.18294574297705687No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7970.1681750370850223No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7510.15846857321311383No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA7210.15213827068795618No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6440.13589049420671814No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC6370.13441342361751468No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC6310.13314736311248315No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG5840.12322988915640279No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG5660.11943170764130819No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5590.11795463705210472No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5330.11246837486363474No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5320.1122573647794628No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5020.10592706225430513No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT4970.10487201183344552No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA4940.10423898158092976No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG4850.10233989082338245No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4840.10212888073921052No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG4810.10149585048669475No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA4750.10022978998166324No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)