FastQCFastQC Report
Wed 31 May 2023
EGAF00007943558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453906
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT46591.0264239732455618No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC24200.5331500354698991No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA20790.45802434865368596No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG17710.3901688895938807No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA16060.3538177508118421No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC15180.33443047679475485No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG14780.3256180795142607No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG14660.3229743603301124No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14400.31724630209779114No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG14300.3150432027776676No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12770.2813357831797773No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11820.2604063396386036No Hit
TCCTACACCAACGGCAAACTGACTATCACCC10770.23727379677730634No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10710.2359519371852322No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA10320.22735984983675034No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG10280.22647861010870093No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG10230.22537706044863914No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC9750.2148021837120461No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC9440.2079725758196631No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9220.20312575731539131No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9060.19960079840319359No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA8850.19497428983093415No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8430.18572127268641525No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7870.17338391649372337No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7690.16941833771750098No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7660.16875740792146393No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7580.16699492846536507No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7460.1643512092812168No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7120.15686067159279674No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7030.15487788220468554No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6740.14848889417632727No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6670.14694672465224076No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6390.14077804655589485No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6360.14011711675985777No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6230.13725308764369715No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA6190.13637184791564774No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6000.132185959207413No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA5980.13174533934338828No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG5980.13174533934338828No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5970.13152502941137592No Hit
GTAGGCTCCGTGTTCAAAGAAATCATTAACC5710.1257969711790547No Hit
GCGAGCCAGGCCAAAAAAGTAGTTCAGCAGC5500.12117046260679523No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5180.11412054478239987No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG5100.11235806532630105No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5020.11059558587020221No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4980.10971434614215278No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4930.10861279648209102No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT4710.1037659779778192No Hit
AGCCTGGACCAGATTCCGGGCTATCTGAACC4550.10024101906562152No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC4540.10002070913360916No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)