FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007943570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379814
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT52991.3951565766401448No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33920.8930687125803684No Hit
ATGTCCAACATGGACATCGATGGTATTAACA16700.4396889003564903No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA15990.4209955399221725No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT15220.4007224588877714No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG13000.342272796684693No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12960.3412196496179709No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG12260.3227895759503336No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC10960.2885622962818643No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10400.2738182373477544No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG10140.26697278141406056No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9960.262233619613811No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9740.25644131074683923No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC9700.25538816368011713No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9670.2545983033800755No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC9600.25275529601331176No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG9100.23959095767928512No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8510.22405703844513367No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8130.2140521413112734No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG7950.20931297951102382No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG7340.1932524867435113No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6870.18087800870952625No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6770.1782451410427209No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC6730.17719199397599877No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6660.17534898660923504No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG6300.16587066300873587No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6280.1653440894753748No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6000.15797206000831987No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5930.15612905264155613No Hit
TTCGTGGCAAAAGGTGTAGCCGCGGACGCCA5890.155075905574834No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5870.15454933204147292No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG5800.1527063246747092No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG5600.14744058934109855No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5600.14744058934109855No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5360.14112170694076573No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5310.13980527310736307No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG5240.13796226574059933No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG4990.131380096573586No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA4910.12927380244014175No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG4790.12611436123997535No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG4650.12242834650644789No Hit
GAAACCACTATCGCAGATAATAGCAAACGCT4580.12058533913968415No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA4500.1184790450062399No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG4440.1168993244061567No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC4400.11584617733943457No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC4340.11426645673935137No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4230.1113703023058655No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG4050.10663114050561591No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT3930.10347169930544951No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG3920.10320841253876897No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG3920.10320841253876897No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC3880.10215526547204684No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC3800.10004897133860258No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)