FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007943636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462969
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64061.3836779568394428No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT57401.2398238326972215No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA27210.5877283360224982No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA25750.5561927472465759No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC20080.4337223442606309No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC19590.42313848227419115No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC15930.3440835131509885No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC14310.3090919694407185No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC14070.303908037039197No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC13900.30023608492145265No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA13540.2924601863191704No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13520.29202819195237695No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13350.28835623983463254No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA13210.2853322792670784No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12400.26783650741194337No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA11280.24364482287150976No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG9450.20411733830990844No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC9430.203685343943115No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9200.19871740872499022No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA8750.18899753547213743No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT8080.17452572418455664No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7780.16804580868265478No Hit
GTGGCCCCGCTGCGTGCGTCCATGGGTCCGG7600.16415785938151367No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7510.16221388473094311No Hit
TTCTCCCAGGTTGCTGACGTAGTCCGTGCAC7050.1522780142946936No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6950.15011804246072633No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG6810.1470940818931721No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6630.143206132592031No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6500.14039816920787354No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6450.13931818329088988No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6160.13305426497238476No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5730.12376638608632544No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5620.12139041706896142No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA5520.11923044523499413No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT5400.1166384790342334No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5110.11037456071572828No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5080.10972656916553808No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA5070.10951057198214136No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4820.10411064239722315No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA4740.10238266493004931No Hit
GCAGCTCGTGAAGAAGAAGAACCGCACGAAC4720.10195067056325585No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)