FastQCFastQC Report
Wed 31 May 2023
EGAF00007943645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486029
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA20790.4277522534663569No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA18390.3783724839464312No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC16740.34442389240148225No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT16690.34339514720315045No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT15500.31891101148285395No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA13910.2861969141759031No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12730.2619185274952729No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG10500.21603649164967523No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10500.21603649164967523No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG10380.21356750317367895No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9830.20225130599202928No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG9630.19813632519870214No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT9630.19813632519870214No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG9570.196901830960704No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9040.18599713185838704No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT9030.18579138281872068No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC8970.18455688858072256No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG8830.18167640202539354No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG8620.17735567219240003No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT6750.13888060177479122No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG6650.13682311137812764No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6260.1287988988311397No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6010.12365517283948078No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA6010.12365517283948078No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT5890.12118618436348448No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5850.12036318820481905No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT5660.11645395645115827No Hit
GGTACGCTCGACGTGGCAACGTGCCAGCAGCAGACGCAGACCTT5590.11501371317349376No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5580.11480796413382742No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG5450.11213322661816476No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5380.11069298334050026No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5350.11007573622150119No Hit
TGGACGACCGCCGCCGCGACCACGACCGCGGCCACGACCGCGAC5300.10904699102316939No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5140.10575500638850768No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)