FastQCFastQC Report
Wed 31 May 2023
EGAF00007943656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485881
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT81901.6855979138924961No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28810.5929435396732945No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC25530.5254372984331555No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17390.3579065655993957No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG15830.3257999386681101No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC15330.3155093531132109No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG13320.2741411991825159No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13300.2737295757603199No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA12920.2659087307385965No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12690.26117506138334284No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12610.25952856769455895No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12510.2574704505835791No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC11830.2434752542289161No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11790.24265200738452422No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11570.23812414974036852No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10610.21836622547496196No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10320.2123976858531204No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA10290.21178025071982645No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG9840.20251872372041715No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9830.20231291200931917No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9270.190787456187832No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC9050.18625959854367632No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG8790.18090849405512874No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8530.17555738956658112No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG8090.16650167427826978No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG7820.16094475807862418No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7720.15888664096764432No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG7700.15847501754544835No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7690.1582692058343504No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7580.15600527701227254No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7570.15579946530117456No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7570.15579946530117456No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA7330.15085998423482294No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7010.1442740094796874No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6780.13954034012443375No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG6700.1378938464356499No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG6690.1376880347245519No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6540.13460085905808214No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6320.13007300141392647No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5940.12225215639220302No Hit
CTGGCATACCGTTCTCTGACCCGCAAAAAAC5690.1171068636147534No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5600.11525455821487153No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5580.11484293479267556No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5510.11340225281498968No Hit
GCCGTCGCGAACAGCAACAACGACACCGCTA5470.11257900597059774No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5440.11196157083730378No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG5230.10763952490424611No Hit
ACGGCAGCGACTTCCAAAGCCGTTGCTGCCA5210.10722790148205014No Hit
ATGTCTGACGAAGGCCCGGGTACCGGCCCGG5210.10722790148205014No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5190.10681627805985416No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5150.10599303121546222No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5130.10558140779326625No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA5020.10331747897118843No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5000.10290585554899245No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT4940.10167098528240454No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC4910.10105355014911059No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA4910.10105355014911059No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)