FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007943682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489364
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49471.0109039487988492No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT36050.73667045389526No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC31350.6406274266190403No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG27600.5639973516646096No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19460.39765900229685874No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT18930.3868286183699659No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18720.3825373341725178No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG14490.29609860962392004No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12980.2652422327756026No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12590.2572727049803418No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC11430.2335684684611046No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT10050.20536860087787415No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10010.20455121341169358No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9700.19821646054879397No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9150.18697738288881077No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8570.17512526462919217No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8450.17267310223065038No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8300.16960789923247316No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8200.16756443056702167No Hit
GCCGAGGGTCTGAAGGACGCGATTAAAGACC8140.1663383493677508No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8090.16531661503502504No Hit
CGTTCTCGCCACGAGAACACCTCTCAGGTTC7730.1579601278393997No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7550.15428188424158704No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7380.1508079875103195No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT7230.1477427845121423No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7120.14549496898014566No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG7000.14304280658160387No Hit
CATGGTGAAGGCTTCCTGAGCGAGGGTAAAG6870.14038629731651694No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6640.13568631938597853No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6520.13323415698743676No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5970.1219950793274536No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5640.11525163273146369No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5400.11034730793438013No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5320.10871253300201895No Hit
GCTCCGACCTGCAACATCCGTGTTACCGTTT4930.10074300520675815No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC4920.100538658340213No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)