FastQCFastQC Report
Sun 21 May 2023
EGAF00007943851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505510
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT31440.6219461533896461No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA21070.41680678918320113No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT20740.4102787284128899No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT19590.3875294257284722No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA18580.3675496033708532No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT18510.36616486320745384No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC17660.349350161223319No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC14330.283476093450179No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT13430.2656722913493304No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA10560.20889794464995748No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA10530.20830448457992917No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA10420.2061284643231588No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG10320.20415026408973114No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT10050.19880912345947654No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT9660.19109414254910884No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT9550.18891812229233843No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG9540.18872030226899567No Hit
GCGCTGTTTAGCCAGCAGTTCCGCTGCTTTACGTTCTTCCTGCT9230.18258788154537No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8790.17388380051828847No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC8400.1661688196079207No Hit
TACGGTATCAGGGGATTCCGGCTGCACCTCGGTTTCGCCTTTGT7960.15746473858083915No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA7830.15489307827738324No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7830.15489307827738324No Hit
GAACACTACAACTTTTTTGTTGCCGATCAGATAAATACCGGCCG7620.15073885778718524No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG7360.14559553718027338No Hit
CTGTTCGGTTTCCAGTTCGCCTACGCTCACTTTGGTGTTGTTAC6930.1370892761765346No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6670.13194595556962277No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA6540.12937429526616684No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT6490.128385195149453No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT6260.12383533461256949No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG6150.1216593143557991No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6000.11869201400565765No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5850.1157247136555162No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5660.11196613321200373No Hit
TTGACCACGCTGCTGCGGCTGCTGCGGTTCTTCGAAAGTGCTCG5630.11137267314197544No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT5610.11097703309528992No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC5450.10781191272180571No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5370.1062293525350636No Hit
TTCTGCGGAGTTCGGTACGATTTCCAGCTGCGGAACTTTATATT5210.1030642321615794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)