FastQCFastQC Report
Wed 31 May 2023
EGAF00007943862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515247
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA53881.0457120565476363No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA24860.4824870401962554No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20650.4007786556738807No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA19060.3699196695953591No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC18350.35613987078042186No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14660.2845237332774378No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC13210.2563818906272137No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC13120.25463515556616534No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11010.2136839224682531No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10670.2070851455709592No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9860.19136453002152365No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9340.18127228300213297No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA9290.18030187463488387No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8680.16846289255444477No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8180.1587588088819537No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7560.1467257451280648No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT7060.13702166145557373No Hit
TCCTACACCAACGGCAAACTGACTATCACCC7030.13643941643522425No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC6540.12692941443618305No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6510.12634716941583357No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6450.12518267937513464No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6250.12130104590613823No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC6240.1211069642326884No Hit
GTTACCCTGGATGCAGGTAACCGTTTCTCTA6140.11916614749819018No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG6110.11858390247784073No Hit
GAAATGGAGAACATCCTGCAGAACCTGACTC5620.1090739004787995No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG5550.10771532876465074No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5550.10771532876465074No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5540.10752124709120091No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT5340.10363961362220449No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)