FastQCFastQC Report
Sun 21 May 2023
EGAF00007943869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404164
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA91522.2644273116853553No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA90852.2478498827208755No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC44401.098563949287913No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA36100.8932017695786859No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA30980.7665205213725121No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA26770.6623548856404825No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC25410.6287051790857177No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT23970.5930760780277313No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA21740.537900456250433No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT21130.5228075731633693No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA16420.4062707217862056No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG16090.3981057194604171No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG14230.352084797260518No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT13920.3444146435605348No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT13290.3288269118476658No Hit
TTCAGACGGTGGGGTGTCCTCGGACTGGCCCCAGTCGCTCGGGC10440.25831098267040115No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG10150.2511356775962233No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA9820.24297067527043478No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT9160.2266406706188577No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG8680.21476430359952892No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT8630.21352718203501547No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA8330.20610445264793498No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG8060.19942399619956255No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC7640.18903217505764985No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG7400.18309399154798547No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6790.1680011084609218No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA6270.15513504418998228No Hit
GGGCACTATCGAGCAACGCCTTCATTGCATCCGAGTAAGTATGA6250.15464019556417694No Hit
ACCGCCCAGAGAATCAGCGATCAGATGGGAACGGTCGAACAGGT5990.14820716342870716No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG5920.14647519323838837No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT5710.14127928266743203No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC5480.13558852347067032No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5430.1343514019061569No Hit
GTCAATCAGGGTACAGTTTTTACCATCCAGGATGCGGTGCGGAG5250.12989776427390862No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5040.12470185370295228No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG5010.12395958076424421No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC4950.12247503488682812No Hit
CGGCTGACCGTCATCTGCTACATTGGTACAGACGCGTGCGCCGT4940.12222761057392544No Hit
GGTACCGCTGTTCAGGGTGTACTGCTGGAACAGCTGCTTCCACG4940.12222761057392544No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG4750.11752654862877447No Hit
GATGCGATGCGGGGAGTCGCAGATACGACCGGTAGAGCTGGACT4740.11727912431587179No Hit
TTTCACGTGTTCATACTTAGAAACATCGAAACCGTCCTGTTTGG4660.11529972981265031No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT4640.11480488118684494No Hit
CTCTGGATTTAAATATGTCTATCAGGTGGACGGGCCTTCACCTG4640.11480488118684494No Hit
CGGGCCTTCACCTGGATCGTCCGCCGGACCCTGTTCGATAGCAC4430.1096089706158886No Hit
CAGTGCATCGATCAGAGTACAGTTTTTGCCATCCAGGATACGAT4370.1081244247384725No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT4250.1051553329836403No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG4210.10416563573202957No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)