FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007943875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381555
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA23370.6124936116680426No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA20120.5273158522362438No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT17370.4552423634862602No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT16660.43663429911808255No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC14530.3808101060135498No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG13950.3656091520226442No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT12540.3286551086999253No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA12170.31895794839538205No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT12150.3184337775681095No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT11440.29982571319993184No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT9780.2563195345363054No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9380.24583611799085323No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG9270.2429531784408539No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT9040.23692521392721888No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7730.20259202474086305No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7420.19446737691813762No Hit
GACCGGCCATTCCGGGGTGCGCGGACGTGGACGACCGTGAATAG7200.18870149781813894No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC6930.18162519164995872No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA6800.17821808127268676No Hit
TTGTGGCACCGGTGGCAGCAGGGTGGCCATGACACGCTGTTCCA6740.17664556879086896No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT6550.17166594593177917No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA6520.17087968969087025No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA6340.16616215224541678No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG5590.14650574622269397No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT5480.14362280667269464No Hit
CATGCTGTCCTGCAGCGGCATCGGTGGTGGCGGGAAGCGGGTAG5480.14362280667269464No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5350.1402156962954227No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA4660.1221318027545177No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG4630.12134554651360878No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT4620.12108346109997248No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC4590.12029720485906358No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT4520.11846260696360944No Hit
CCACTGTTCCGGAACCAGTGGACCTTCCATCGGCTGCTGAGGCA4520.11846260696360944No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT4450.11662800906815532No Hit
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG4190.10981378831361141No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT3970.10404790921361273No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)