FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007943916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504890
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT117742.331993107409535No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG57971.1481708887084316No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG29730.5888411337122937No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA27820.5510111113311811No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC21250.42088375685792945No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC20410.40424646952801596No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC18000.3565132999267167No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC17610.34878884509497116No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC16780.33234962070946145No Hit
CAGCCGCAGCGCCGTAAACGTCGTGCGGCTC16570.3281902988769831No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC15810.3131375151022995No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC13800.2733268632771495No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12780.2531244429479689No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12730.2521341282259502No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC12470.2469844916714532No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT12370.24500386222741588No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA11910.23589296678484423No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10990.21767117589970095No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA10790.2137099170116263No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9700.19212105607161958No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9630.19073461546079343No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9350.18518885301748894No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA8500.1683535027431718No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8210.16260967735546358No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG7250.14359563469270534No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7160.14181306819307174No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA7150.14161500524866802No Hit
GCGGAGCAGCACACGCCGATGAAGGCGCACG7090.14042662758224564No Hit
GCACCGAGCGTTGATCCAATCGTGGAGGCTA7070.14003050169343817No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6730.1332963615837113No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6530.12933510269563667No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT6300.12477965497435085No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6160.12200677375269861No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5900.1168571371982016No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG5730.11349006714333815No Hit
GCGAACGAAGCAGGCCAGGTGGCCGGCGGTG5710.11309394125453068No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5660.11210362653251203No Hit
GTTACCCTGGATGCAGGTAACCGTTTCTCTA5650.1119055635881083No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG5640.11170750064370458No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5570.11032106003287843No Hit
CAGACTGTTACCTCTACCCCGGTCCAGGGCC5520.10933074531085979No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5470.10834043058884114No Hit
GCGCTGAAGGCGGGCGCAGTTGAAGCGGCGT5430.10754817881122622No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5370.10635980114480383No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT5220.10338885697874785No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)