FastQCFastQC Report
Sun 21 May 2023
EGAF00007943920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007943920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510613
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA107812.111383768137513No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA56011.0969168430886014No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA53021.0383597754072067No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG23880.46767316930826286No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC23460.45944776180786623No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21820.42732950394917485No Hit
GAATGCGACAACTATCGCCAGGAATACGATA20810.4075493573410783No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG20630.4040241826980512No Hit
ACTCCGTCTGACGCAGGCGAAACGGTCGCGG20130.39423203091186476No Hit
GCTAAAACCGCAGATACCCCGGCGACCTCTA13920.2726135057274296No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13700.2683049589415076No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC13360.2616462957269008No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT13050.25557516161946525No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA12540.24558716679755507No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG9860.19310123322359596No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC9800.19192617500925357No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9790.19173033197352984No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9570.18742178518760785No Hit
ATCGAAGGTACTGACGATAAATGCGAGGAAG9510.18624672697326547No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA9320.18252570929451464No Hit
TCCTACACCAACGGCAAACTGACTATCACCC9140.17900053465148752No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT8820.17273355750832822No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG8670.1697959119724723No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8640.16920838286530113No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG8490.1662707373294452No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8390.16431230697220792No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8300.16254971965069437No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG7840.15354094000740287No Hit
ATGCAGCAGCAGGCATACATTAACGCGACCA7590.14864486411430966No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7420.1453155325070063No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7320.143357102149769No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6850.1341524794707538No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG6420.12573122893463345No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG6250.12240189732733009No Hit
GTTGAAACCCCGCAGCCGACCGCTGTATCCG6020.11789750750568434No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5840.11437233286265723No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5690.11143468732680133No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT5570.10908457089811657No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC5520.10810535571949793No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG5310.10399265196929963No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5230.10242590768350981No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5160.10105500643344373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)